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Phospho-NF-kB p65/RelA-S529 Rabbit Polyclonal Antibody (CABP0944)

SKU:
CABP0944
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
€419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-NF-kB p65/RelA-S529 Rabbit Polyclonal Antibody
SKU:CABP0944
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S529 of human NF-kB p65/RelA (NP_068810.3).
Sequence:LLSG D
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:p65; CMCU; NFKB3; AIF3BL3; Phospho-NF-kB p65/RelA-S529
Positive Sample:HeLa,NIH/3T3
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:60kDa
Observed MW:57kDa

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Purification Method:Affinity purification
Gene ID:5970
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using Phospho-NF-kB p65/RelA-S529 antibody (CABP0944) at 1:1000 dilution.HeLa cells were treated by both TNF-α(20ng/mL) and Calyculin A (100nM) for 10 minutes.NIH/3T3 cells were treated by TNF-α(20ng/mL) for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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