In a similar vein, the Phospho-RelA (S529) Rabbit Polyclonal Antibody (CABP0944) is a powerful tool for researchers studying the transcription factor RelA and its phosphorylation at serine 529. This antibody, also raised in rabbits, is highly specific to human samples and is optimized for use in Western blotting applications. By targeting the phosphorylated form of RelA, this antibody allows for the precise detection and analysis of RelA activation in various cell types.RelA is a key player in the NF-kB signaling pathway, which controls many aspects of immune and inflammatory responses. The phosphorylation of RelA at serine 529 is known to regulate its transcriptional activity and impact gene expression related to inflammation and cell survival.
Therefore, studying this post-translational modification of RelA is essential for understanding the intricate mechanisms of immune response and inflammatory diseases.With its high specificity and sensitivity, the Phospho-RelA (S529) Rabbit Polyclonal Antibody (CABP0944) is an invaluable tool for research in immunology, cancer biology, and inflammation. By enabling the precise detection of phosphorylated RelA, this antibody facilitates the investigation of signaling pathways involved in immune regulation and provides insights that can drive the development of innovative therapies for various inflammatory and autoimmune disorders.
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5970
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Phospho-NF-kB p65/RelA-S529 antibody (CABP0944) at 1:1000 dilution.HeLa cells were treated by both TNF-α(20ng/mL) and Calyculin A (100nM) for 10 minutes.NIH/3T3 cells were treated by TNF-α(20ng/mL) for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
