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Phospho-NF-kB p65/RelA-S468 Rabbit Polyclonal Antibody (CABP0446)

SKU:
CABP0446
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
€419
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Description

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Product Name:Phospho-NF-kB p65/RelA-S468 Rabbit Polyclonal Antibody
SKU:CABP0446
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Rat
Immunogen:A synthetic phosphorylated peptide around S468 of human Phospho-NF-kB p65/RelA-S468 (NP_001138610.1).
Sequence:VDNS E
Tested Applications:WB ELISA
Recommended Dilution:WB,1:100 - 1:500
Synonyms:p65; CMCU; NFKB3; AIF3BL3; Phospho-NF-kB p65/RelA-S468
Positive Sample:Hela,C6
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:60kDa
Observed MW:60kDa

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Purification Method:Affinity purification
Gene ID:5970
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from Hela cells, using Phospho-NF-kB p65/RelA-S468 Rabbit pAb (CABP0446) at 1:400 dilution.Hela cells were treated by TNF-α (20 ng/ml) and Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

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