The Phospho-RelA (S311) Polyclonal Antibody (CABP0445) is a valuable tool for researchers studying the phosphorylation of the RelA protein, a key player in the NF-κB signaling pathway. This antibody, raised in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. By specifically recognizing the phosphorylated form of RelA at serine 311, this antibody allows for the detection and analysis of NF-κB signaling activation in various cell types.RelA, also known as p65, is a critical component of the NF-κB complex that regulates gene expression in response to various stimuli, including inflammation, immune responses, and cell survival. Phosphorylation of RelA at serine 311 has been linked to its transcriptional activity and is a key event in the regulation of NF-κB-mediated gene expression.
Understanding the phosphorylation status of RelA is essential for deciphering the complex regulatory mechanisms of NF-κB signaling and its implications in diseases such as cancer, inflammatory disorders, and autoimmune diseases.Overall, the Phospho-RelA (S311) Polyclonal Antibody is a valuable tool for researchers aiming to unravel the intricacies of NF-κB signaling and its role in various physiological and pathological processes. Its specificity for phosphorylated RelA at serine 311 enables precise detection and quantification of NF-κB activation, making it
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5970
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of HeLa, using Phospho-NF-kB p65/RelA-S311 antibody (CABP0445) at 1:1000 dilution.HeLa cells were treated by TNF-α (20 ng/ml) and Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
