The Phospho-RelA (S276) Polyclonal Antibody (CABP0123) is a vital tool for researchers studying the phosphorylation of the RelA protein, a key regulator of NF-κB signaling pathways. This antibody specifically targets the phosphorylated serine 276 residue of RelA, allowing for the detection and analysis of protein activation in various cell types.RelA, also known as p65, is a critical component of the NF-κB transcription factor complex, which plays a crucial role in regulating immune responses, inflammation, and cell survival. Phosphorylation of RelA at serine 276 is known to influence its transcriptional activity, making this antibody essential for understanding the signaling mechanisms involved in these processes.
The Phospho-RelA (S276) Polyclonal Antibody is raised in rabbits and validated for use in Western blot applications, providing reliable and accurate results for researchers investigating the role of NF-κB signaling in various biological processes. With its high reactivity with human samples, this antibody is an invaluable tool for studies in immunology, cancer research, and inflammatory diseases.
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5970
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-NF-kB p65/RelA-S276 Rabbit pAb (CABP0123) at 1:400 dilution.HeLa cells were treated by TNF-α (20 ng/ml) and Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
