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Phospho-RB-S608 Rabbit Polyclonal Antibody (CABP0570)

SKU:
CABP0570
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
€419
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Description

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Product Name:Phospho-RB-S608 Rabbit Polyclonal Antibody
SKU:CABP0570
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around S608 of human RB (NP_000312.2).
Sequence:YLSP V
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:RB; pRb; OSRC; pp110; p105-Rb; PPP1R130; p110-RB1; Phospho-RB-S608
Positive Sample:Jurkat
Conjugate:Unconjugated
Cellular Localization:Nucleus.
Calculated MW:106kDa
Observed MW:110kDa

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

Purification Method:Affinity purification
Gene ID:5925
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of Jurkat cells, using Phospho-RB-S608 antibody (CABP0570) at 1:2000 dilution. Jurkat cells were treated by 10% FBS for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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