The Phospho-Rb (S811) Polyclonal Antibody (CABP0089) is a valuable tool for researchers studying the phosphorylation of the Retinoblastoma (Rb) protein at serine 811. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the phosphorylated form of Rb at serine 811, this antibody allows for the detection and analysis of this critical marker in various cell types.The phosphorylation of Rb at serine 811 plays a key role in regulating cell cycle progression, proliferation, and differentiation. Dysregulation of this phosphorylation event has been implicated in various diseases, including cancer.
Therefore, understanding the role of phospho-Rb (S811) is essential for unraveling the mechanisms underlying cancer development and progression.Researchers studying cell cycle control, cell proliferation, and cancer biology will find the Phospho-Rb (S811) Polyclonal Antibody to be a valuable asset in their research endeavors. Its specificity for the phosphorylated form of Rb at serine 811 makes it an ideal tool for investigating the intricate regulatory pathways governing cell growth and division.
Product Name:
Phospho-RB-S811 Rabbit Polyclonal Antibody
SKU:
CABP0089
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S811 of human RB (NP_000312.2).
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.
Purification Method:
Affinity purification
Gene ID:
5925
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa cells, using Phospho-RB-S811 antibody (CABP0089) at 1:1000 dilution. HeLa cells were treated by UV for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
