Based on the information provided in the URL, the Phospho-Rb (S807/811) Polyclonal Antibody (CABP0484) is a valuable tool for researchers studying cell cycle regulation and cancer biology. This antibody, raised in rabbits, specifically targets the phosphorylated form of the Retinoblastoma protein (Rb) at serine 807 and 811 residues. It is highly reactive with human samples and has been validated for use in Western blot applications.Rb is a key tumor suppressor protein that plays a crucial role in controlling cell proliferation and differentiation. Phosphorylation of Rb at serine 807 and 811 regulates its activity, allowing for progression through the cell cycle.
Aberrant phosphorylation of Rb has been implicated in various types of cancer, making it a promising target for therapeutic intervention.By using the Phospho-Rb (S807/811) Polyclonal Antibody, researchers can effectively study the phosphorylation status of Rb in different cell types and under various experimental conditions. This antibody enables the detection and analysis of phosphorylated Rb, providing valuable insights into the mechanisms underlying cell cycle progression and cancer development. Overall, this antibody is a valuable tool for advancing research in the fields of cell biology and cancer therapeutics.
Product Name:
Phospho-RB-S807/811 Rabbit Polyclonal Antibody
SKU:
CABP0484
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S807 & S811 of human RB (NP_000312.2).
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.
Purification Method:
Affinity purification
Gene ID:
5925
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of HT-29 cells, using Phospho-RB-S807/811 antibody (CABP0484) at 1:1000 dilution. HT-29 cells were treated by Nocodazole (100ng/mL) for 16 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
