The Phospho-RB (S795) Polyclonal Antibody (CABP0088) offered by AssayGenie is a valuable tool for researchers studying cell cycle regulation and cancer biology. This antibody, generated in rabbits, specifically recognizes phosphorylated RB at serine 795 and is validated for use in Western blot applications. RB, a tumor suppressor protein, is a key regulator of cell cycle progression and proliferation. Phosphorylation at serine 795 is associated with RB inactivation and promotes cell cycle progression, making it a crucial site for understanding cancer development and progression.
This antibody enables the detection and analysis of phosphorylated RB in various cell types, providing insights into the molecular mechanisms underlying tumorigenesis. It is an essential tool for researchers investigating the role of RB phosphorylation in cancer signaling pathways and developing targeted therapies for RB-associated malignancies.
Product Name:
Phospho-RB-S795 Rabbit Polyclonal Antibody
SKU:
CABP0088
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S795 of human RB (NP_000312.2).
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.
Purification Method:
Affinity purification
Gene ID:
5925
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from 293 cells, using Phospho-RB-S795 Rabbit pAb (CABP0088).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
