The Phospho-Rb (S780) Rabbit Monoclonal Antibody (CABP0117) is a valuable tool for researchers studying the phosphorylation of the retinoblastoma protein, a key regulator of cell cycle progression and tumor suppression. This antibody is specifically designed to detect phosphorylation at serine 780 of the retinoblastoma protein, allowing for precise analysis of signaling pathways involved in cell growth and proliferation.Raised in rabbits, this monoclonal antibody demonstrates high reactivity with human samples and has been validated for use in Western blotting applications. By detecting phospho-Rb at serine 780, researchers can gain insights into the activation or inhibition of cell cycle regulators, providing valuable information for studies in cancer biology, cell signaling, and drug development.
The Phospho-Rb (S780) Rabbit Monoclonal Antibody is an essential tool for investigating the role of phosphorylation in the regulation of cell cycle progression and tumor development. Its ability to specifically target phosphorylated serine 780 of the retinoblastoma protein makes it a reliable choice for researchers seeking to understand the molecular mechanisms underlying cancer and potential therapeutic targets for intervention.
Product Name:
Phospho-RB-S780 Rabbit Monoclonal Antibody
SKU:
CABP0117
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S780 of human RB (P06400).
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.
Purification Method:
Affinity purification
Gene ID:
5925
Clone Number:
ARC0096
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells, using Phospho-RB-S780 Rabbit mAb (CABP0117) at 1:1000 dilution.C6 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
