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Phospho-RB-S780 Rabbit Monoclonal Antibody (CABP0117)

SKU:
CABP0117
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cell Cycle
€419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-RB-S780 Rabbit Monoclonal Antibody
SKU:CABP0117
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S780 of human RB (P06400).
Sequence:TLSP IP
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:500 - 1:2000 IHC-P,1:50 - 1:200
Synonyms:RB; pRb; OSRC; pp110; p105-Rb; PPP1R130; p110-RB1; Phospho-RB-S780
Positive Sample:C6+serum
Conjugate:Unconjugated
Cellular Localization:Nucleus.
Calculated MW:106kDa
Observed MW:106kDa

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

Purification Method:Affinity purification
Gene ID:5925
Clone Number:ARC0096
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from C6 cells, using Phospho-RB-S780 Rabbit mAb (CABP0117) at 1:1000 dilution.C6 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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