The Phospho-Rb (S780) Monoclonal Antibody (CABP1419) is a cutting-edge tool for researchers studying the phosphorylation of the retinoblastoma protein (Rb) at serine 780. This antibody, produced through a rigorous process in mouse cells, is highly specific for detecting phosphorylated Rb in human samples and is validated for use in Western blot applications.The phosphorylation of Rb at serine 780 is known to regulate cell cycle progression and is implicated in various diseases, including cancer. This antibody enables precise detection and analysis of phosphorylated Rb in different cell types, making it a valuable asset for studies in cell biology, cancer research, and drug development.
Understanding the phosphorylation status of Rb at serine 780 is crucial for unraveling the molecular mechanisms underlying cell cycle regulation and disease progression. The Phospho-Rb (S780) Monoclonal Antibody (CABP1419) provides researchers with a reliable tool to investigate the role of phosphorylated Rb in health and disease, paving the way for potential therapeutic interventions targeting this critical signaling pathway.
Product Name:
Phospho-RB-S780 Monoclonal Antibody
SKU:
CABP1419
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A phospho synthetic peptide corresponding to residues surrounding S780 of Human RB.
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.
Purification Method:
Affinity purification
Gene ID:
5925
Clone Number:
ARC52461
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-RB-S780 Rabbit mAb (CABP1419) at 1:19000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
