Raf1 Rabbit Polyclonal Antibody (CAB0223)
- SKU:
- CAB0223
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
Product Name: | Raf1 Rabbit Polyclonal Antibody |
SKU: | CAB0223 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 190-350 of human Raf1 (NP_002871.1). |
Sequence: | IRQL LLFP NSTI GDSG VPAL PSLT MRRM RESV SRMP VSSQ HRYS TPHA FTFN TSSP SSEG SLSQ RQRS TSTP NVHM VSTT LPVD SRMI EDAI RSHS ESAS PSAL SSSP NNLS PTGW SQPK TPVP AQRE RAPV SGTQ EKNK IRPR GQRD SSYY WEIE ASEV M |
Tested Applications: | WB IHC-P IF/ICC ELISA |
Recommended Dilution: | WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 |
Synonyms: | NS5; CRAF; Raf-1; c-Raf; CMD1NN; Raf1 |
Positive Sample: | Mouse liver |
Conjugate: | Unconjugated |
Cellular Localization: | Cell membrane, Cytoplasm, Mitochondrion, Nucleus. |
Calculated MW: | 73kDa |
Observed MW: | 73kDa |
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Purification Method: | Affinity purification |
Gene ID: | 5894 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |