The Phospho-RAF1 (S621) Rabbit Monoclonal Antibody (CABP1011) is a valuable tool for researchers studying the RAF1 protein and its phosphorylation at serine 621. RAF1 is a key player in the MAPK signaling pathway, which is involved in cell proliferation, differentiation, and survival. Phosphorylation of RAF1 at serine 621 is known to activate its kinase activity, leading to downstream signaling events.This antibody, generated in rabbits, shows high specificity and sensitivity in detecting phosphorylated RAF1 at serine 621 in human samples. It is validated for use in Western blot applications, allowing for precise analysis of RAF1 phosphorylation levels in various cell types and tissues.
This makes it an ideal choice for studies in cell signaling, cancer research, and drug development targeting the MAPK pathway.Understanding the regulation of RAF1 phosphorylation at serine 621 is crucial for uncovering its role in different diseases, including cancer and neurological disorders. By using the Phospho-RAF1 (S621) Rabbit Monoclonal Antibody, researchers can gain valuable insights into the mechanisms underlying RAF1 activation and its implications in disease pathology.
Product Name:
Phospho-Raf1-S621 Rabbit Monoclonal Antibody
SKU:
CABP1011
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic phosphorylated peptide around S621 of human Phospho-Raf1-S621 (P04049).
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Purification Method:
Affinity purification
Gene ID:
5894
Clone Number:
ARC1556
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of C6 cells, using Phospho-Raf1-S621 Rabbit mAb (CABP1011) at 1:1000 dilution.C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
