The Phospho-RAF1 (S621) Polyclonal Antibody (CABP0087) is a valuable tool for researchers studying the RAF1 protein and its phosphorylation at serine 621. This antibody, raised in rabbits, is highly specific to the phospho-RAF1 (S621) protein in human samples and is validated for use in Western blot applications.Phospho-RAF1 (S621) is a critical component of the MAPK/ERK signaling pathway, which regulates cell growth, proliferation, and survival. Phosphorylation of RAF1 at serine 621 is known to enhance its kinase activity, leading to downstream activation of signaling cascades involved in cancer progression and other cellular processes.
By using the Phospho-RAF1 (S621) Polyclonal Antibody, researchers can detect and analyze the phosphorylation status of RAF1 in various cell types, providing insights into its role in cancer and other diseases. Understanding the regulation of RAF1 phosphorylation at serine 621 is essential for unraveling its complex signaling network and developing targeted therapies for diseases associated with dysregulated MAPK/ERK signaling.
Product Name:
Phospho-Raf1-S621 Rabbit Polyclonal Antibody
SKU:
CABP0087
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S621 of human Phospho-Raf1-S621 (NP_002871.1).
Sequence:
SASE P
Tested Applications:
WBIPELISA
Recommended Dilution:
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Purification Method:
Affinity purification
Gene ID:
5894
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa and 293T cells, using Phospho-Raf1-S621 Rabbit pAb (CABP0087) at 1:1000 dilution. HeLa cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. 293T cells were treated by PMA/TPA (200nM) for 30 minutes or treated by EGF (25μg/mL) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
