The Phospho-RAF1 (S259) Rabbit Monoclonal Antibody (CABP1012) is a cutting-edge tool for researchers investigating the phosphorylation of RAF1 at serine 259, a crucial event in cell signaling pathways and cancer development. This highly specific antibody, produced in rabbits, exhibits strong reactivity with human samples and is suitable for Western blot applications. By targeting the phosphorylated form of RAF1, this antibody enables precise detection and analysis of this important protein modification in various cell types, making it an essential tool for studies in cancer biology and signal transduction research.RAF1 is a key effector kinase in the RAS-RAF-MEK-ERK signaling pathway, which plays a central role in cell proliferation, survival, and differentiation.
Phosphorylation of RAF1 at serine 259 is known to regulate its activity and downstream signaling, making it a critical event in the control of cell growth and transformation. Understanding the phosphorylation status of RAF1 can provide important insights into the mechanisms underlying oncogenesis and may offer potential therapeutic targets for cancer treatment.The Phospho-RAF1 (S259) Rabbit Monoclonal Antibody (CABP1012) offers researchers a valuable tool for investigating the role of RAF1 phosphorylation in cancer development and other cellular processes. Its high specificity and sensitivity make it a reliable choice for detecting and studying this critical protein modification, opening up new opportunities for exploring the
Product Name:
Phospho-Raf1-S259 Rabbit Monoclonal Antibody
SKU:
CABP1012
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic phosphorylated peptide around S259 of human RAF1 (P04049).
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Purification Method:
Affinity purification
Gene ID:
5894
Clone Number:
ARC1557
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Phospho-Raf1-S259 Rabbit mAb (CABP1012) at 1:1000 dilution.Both 293T cells and C6 cells were treated by Forskolin (10 uM) at 37℃ for 1 hour after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
