The Phospho-Raf1 (S259) Polyclonal Antibody (CABP0497) is a valuable tool for researchers studying the Raf1 protein, a key player in the MAPK signaling pathway. This antibody is raised in rabbits and exhibits high reactivity with human samples, making it suitable for a range of applications, including Western blotting. By specifically binding to the phosphorylated form of Raf1 at serine 259, this antibody allows for precise detection and analysis of Raf1 activation in various cell types.Raf1 is a crucial kinase involved in cell growth, differentiation, and survival, making it a potential target for cancer research and drug development. Phosphorylation at serine 259 plays a role in regulating Raf1 activity and downstream signaling cascades, highlighting the importance of studying this post-translational modification.
Understanding the phosphorylation status of Raf1 can provide valuable insights into aberrant signaling pathways associated with diseases like cancer, cardiovascular disorders, and neurodegenerative diseases.Overall, the Phospho-Raf1 (S259) Polyclonal Antibody is a reliable tool for investigating the role of Raf1 phosphorylation in cellular signaling pathways, offering new opportunities for therapeutic intervention and biomarker discovery in various disease contexts.
Product Name:
Phospho-Raf1-S259 Rabbit Polyclonal Antibody
SKU:
CABP0497
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S259 of human Phospho-Raf1-S259 (NP_002871.1).
Sequence:
STST P
Tested Applications:
WBIPELISA
Recommended Dilution:
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Purification Method:
Affinity purification
Gene ID:
5894
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-Raf1-S259 Rabbit pAb (CABP0497) at 1:1000 dilution. HeLa cells were treated by 10% FBS after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
