The Phospho-Rac1 (S71) Polyclonal Antibody (CABP0086) is a powerful tool for researchers studying Rac1, a protein involved in cellular signaling pathways and regulation of the cytoskeleton. This antibody, produced in rabbits, is highly specific for detecting phosphorylation of Rac1 at serine 71 in human samples, making it a valuable tool for investigating Rac1 signaling in various cellular processes.Rac1 is a member of the Rho family of GTPases and plays a crucial role in cellular migration, proliferation, and cytoskeletal dynamics. Phosphorylation of Rac1 at serine 71 has been shown to regulate its activity and function in different cellular contexts, including cancer progression, cell adhesion, and cell motility.
The Phospho-Rac1 (S71) Polyclonal Antibody enables researchers to study the phosphorylation status and activity of Rac1 in response to various stimuli and treatments.This antibody is validated for use in Western blot applications, allowing for the detection and quantification of phosphorylated Rac1 in cell lysates and tissue samples. By targeting the phosphorylated form of Rac1 at serine 71, researchers can gain insight into the role of Rac1 signaling in cancer, cell migration, and other biological processes. This antibody is a valuable tool for studies in cell biology, cancer research, and signal transduction pathways involving Rac1.
Product Name:
Phospho-Rac1/2/3-S71 Rabbit Polyclonal Antibody
SKU:
CABP0086
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic phosphorylated peptide around S71 of human RAC1 (NP_008839.2).
The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5879
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of A-431 cells, using Phospho-Rac1/2/3-S71 antibody (CABP0086) at 1:1000 dilution.A-431 cells were treated by EGF (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
