The Phospho-Rac1/Cdc42 (S71) Rabbit Monoclonal Antibody (CABP1018) is a valuable tool for researchers studying the phosphorylation of Rac1 and Cdc42 proteins. These proteins are key regulators of cell migration, cytoskeletal organization, and cell proliferation. The antibody is produced in rabbits and is highly specific for detecting phosphorylation at serine 71 of Rac1 and Cdc42 in human samples.This antibody is validated for use in Western blot applications, providing researchers with a reliable method to study the activation status of Rac1 and Cdc42 in different cell types. Understanding the phosphorylation of these proteins is essential for investigating their roles in cellular processes such as cell adhesion, migration, and invasion.
Additionally, dysregulation of Rac1 and Cdc42 phosphorylation has been linked to cancer progression and metastasis.Overall, the Phospho-Rac1/Cdc42 (S71) Rabbit Monoclonal Antibody is a valuable tool for researchers in the fields of cell biology, cancer research, and signal transduction. Its high specificity and sensitivity make it an essential reagent for studying the phosphorylation status of Rac1 and Cdc42 proteins in various physiological and pathological conditions.
Product Name:
Phospho-Rac1/Cdc42-S71 Rabbit Monoclonal Antibody
SKU:
CABP1018
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S71 of human Rac1/Cdc42CDC42 (P60953).
Sequence:
PLSY P
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
CDC42Hs; G25K; TKS; Phospho-Rac1/Cdc42-S71
Positive Sample:
A-431
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Cytoplasmic ribonucleoprotein granule, Cytosol, dendritic spine, Extracellular exosome, Filopodium, Focal adhesion, Golgi transport complex, Leading edge membrane, Microtubule organizing center, Mitotic spindle, Phagocytic vesicle, Plasma membrane, Spindle midzone
Calculated MW:
24kDa
Observed MW:
24kDa
The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. [provided by RefSeq, Apr 2013]
Purification Method:
Affinity purification
Gene ID:
998/5879
Clone Number:
ARC1563
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of A-431 cells, using Phospho-Rac1/Cdc42-S71 Rabbit mAb (CABP1018) at 1:1000 dilution.A-431 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.
