The Phospho-PTPN6 (Y564) Polyclonal Antibody (CABP0787) is an essential tool for researchers studying the PTPN6 protein, also known as the SHP1 phosphatase. This antibody, produced in rabbits, is highly specific for human samples and has been validated for Western blot applications. It recognizes the phosphorylated form of the PTPN6 protein at the tyrosine 564 residue, allowing for precise detection and quantification.PTPN6 plays a critical role in immune signaling pathways by negatively regulating immune responses and controlling cell activation. Abnormalities in PTPN6 have been implicated in autoimmune diseases, cancer, and other immune-related disorders.
This antibody enables researchers to study the phosphorylation status of PTPN6, providing valuable insights into the mechanisms underlying these conditions.By utilizing the Phospho-PTPN6 (Y564) Polyclonal Antibody in their research, scientists can better understand the functions of PTPN6 and its role in immune regulation. This knowledge has the potential to lead to the development of targeted therapies for diseases involving dysregulated immune responses.
Product Name:
Phospho-SHP1-Y564 Rabbit Polyclonal Antibody
SKU:
CABP0787
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around Y564 of human PTPN6 (NP_002822.2).
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported.
Purification Method:
Affinity purification
Gene ID:
5777
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa and RAW 264.7 cells, using Phospho-SHP1-Y564 Rabbit pAb (CABP0787) at 1:2000 dilution. HeLa cell lysates were treated by CIP at 37°C for 1 hour.RAW 264.7 cell lysates were treated by CIP at 37°C for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.
