The Phospho-PTPN11 (Y542) Polyclonal Antibody (CABP0267) is a valuable tool for researchers studying PTPN11, a protein involved in cell signaling and regulation. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the phosphorylated form of PTPN11 at tyrosine 542, researchers can study the activation and function of this important signaling molecule in various cell types.PTPN11, also known as SHP2, plays a crucial role in cell development, growth, and differentiation through its involvement in multiple signaling pathways. Dysregulation of PTPN11 activity has been linked to various diseases, including cancer, cardiovascular disorders, and developmental syndromes.
Understanding the phosphorylation status of PTPN11 at tyrosine 542 is essential for unraveling its role in these diseases and identifying potential therapeutic targets for intervention.By using the Phospho-PTPN11 (Y542) Polyclonal Antibody, researchers can gain insights into the signaling mechanisms controlled by PTPN11 and its potential as a target for drug development in the treatment of various diseases. Its high specificity and validation for Western blot applications make it an essential tool for studies in cell biology, cancer research, and drug discovery.
Product Name:
Phospho-SHP2-Y542 Rabbit Polyclonal Antibody
SKU:
CABP0267
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around Y542 of human PTPN11 (NP_002825.3).
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia.
Purification Method:
Affinity purification
Gene ID:
5781
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of NIH/3T3, using Phospho-SHP2-Y542 antibody (CABP0267) at 1:700 dilution.NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
