The Phospho-PTK2B (Y402) Polyclonal Antibody (CABP0214) is a valuable tool for researchers studying the phosphorylation of PTK2B at the Y402 site. PTK2B, also known as focal adhesion kinase 2 (FAK2), is a non-receptor protein tyrosine kinase involved in cell adhesion, migration, and survival pathways.This antibody, produced in rabbits, is highly specific to the phosphorylated form of PTK2B at the Y402 site and is validated for use in Western blot applications. By targeting this specific phosphorylation site, researchers can analyze the activation status of PTK2B in various cellular pathways and processes.
Understanding the regulation of PTK2B phosphorylation at the Y402 site is crucial for unraveling its role in cell signaling and its potential implications in diseases such as cancer, cardiovascular disorders, and neurodegenerative diseases. This antibody offers researchers a reliable tool for investigating the phosphorylation status of PTK2B and its downstream signaling pathways in different experimental settings.
Product Name:
Phospho-PTK2B-Y402 Rabbit Polyclonal Antibody
SKU:
CABP0214
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around Y402 of human PTK2B (NP_004094.3).
This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
2185
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using Phospho-PTK2B-Y402 Rabbit pAb (CABP0214) at 1:1000 dilution.Jurkat cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
