The Phospho-PTEN (S380) Rabbit Polyclonal Antibody (CABP0930) is a vital tool for researchers studying PTEN, a tumor suppressor gene known for its role in regulating cell growth and division. This antibody, raised in rabbits, is highly specific and reacts with human samples, making it ideal for use in Western blot applications. By binding to the phosphorylated form of PTEN at serine 380, this antibody enables precise detection and analysis of PTEN activity in various cell types.PTEN is a critical player in cell signaling pathways that control cell proliferation and survival. Phosphorylation at serine 380 is known to regulate PTEN function and stability, making it an important site for study in cancer research and other related fields.
Understanding the phosphorylation status of PTEN can provide valuable insights into the mechanisms underlying disease progression and may offer new targets for therapeutic intervention.The Phospho-PTEN (S380) Rabbit Polyclonal Antibody (CABP0930) offers researchers a reliable tool for investigating the complex role of PTEN in cancer and other diseases. Its high specificity and sensitivity make it an invaluable asset for studies aiming to unravel the intricate mechanisms of PTEN signaling and its impact on cellular processes. By targeting phosphorylated PTEN at serine 380, this antibody opens up new possibilities for advancing our understanding of PTEN biology and potentially identifying novel therapeutic strategies.
Product Name:
Phospho-PTEN-S380 Rabbit Polyclonal Antibody
SKU:
CABP0930
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S380 of human PTEN (NP_000305.3).
This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
5728
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PTEN-S380 antibody (CABP0930) at 1:1000 dilution.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
