The Phospho-PTEN (S380) Rabbit Monoclonal Antibody (CABP0998) is a valuable tool for researchers studying the PTEN protein, a critical regulator of cell growth and survival. This antibody, generated in rabbits, is highly specific for detecting phosphorylation at serine 380 of PTEN in human samples, making it an ideal choice for Western blot experiments.PTEN, a tumor suppressor gene, is frequently mutated or inactivated in various cancers, leading to uncontrolled cell growth and proliferation. Phosphorylation of PTEN plays a crucial role in regulating its activity, influencing its ability to inhibit cell signaling pathways associated with tumor development.
By using this antibody to detect phospho-PTEN at serine 380, researchers can gain insights into the mechanisms underlying PTEN regulation and its impact on cancer progression.With its high sensitivity and specificity, the Phospho-PTEN (S380) Rabbit Monoclonal Antibody (CABP0998) is a valuable tool for studying PTEN signaling pathways in cancer and other diseases. Its reliability and efficiency make it a valuable asset for researchers seeking to unravel the complexities of PTEN function and potential therapeutic targets related to PTEN dysregulation.
Product Name:
Phospho-PTEN-S380 Rabbit Monoclonal Antibody
SKU:
CABP0998
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S380 of human PTEN (P60484).
This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
5728
Clone Number:
ARC1542
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of 293T cells, using Phospho-PTEN-S380 Rabbit mAb (CABP0998) at 1:1000 dilution.293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
