The Phospho-PRKCQ (T538) Polyclonal Antibody (CABP0798) is a valuable tool for researchers investigating protein kinase C theta (PRKCQ) phosphorylation at threonine 538. PRKCQ is a critical regulator of T cell activation and immune response, making it a key target for studying immune-related diseases and therapies.This antibody, developed using rabbit immunization, exhibits high specificity and sensitivity for detecting phosphorylated PRKCQ in human samples. Validated for Western blot applications, it enables precise measurement and analysis of PRKCQ activation in various cell types.
Phosphorylation at T538 is known to modulate PRKCQ activity and T cell receptor signaling, influencing T cell function and immune responses. Understanding the regulation of PRKCQ phosphorylation provides valuable insights into immune cell activation and could lead to the development of novel immunotherapies for cancer, autoimmune disorders, and inflammatory diseases.Overall, the Phospho-PRKCQ (T538) Polyclonal Antibody is an essential tool for unraveling the complex role of PRKCQ in immune regulation and for advancing research in immunology and related fields.
Product Name:
Phospho-PRKCQ-T538 Rabbit Polyclonal Antibody
SKU:
CABP0798
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around T538 of human PRKCQ (NP_006248.1).
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors.
Purification Method:
Affinity purification
Gene ID:
5588
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat and HeLa cells, using Phospho-PRKCQ-T538 Rabbit pAb (CABP0798) at 1:2000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes.HeLa cells were treated by serum-starvation overnight or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
