The Phospho-PRKCQ (S676) Polyclonal Antibody (CABP0260) is a research tool specifically designed for the investigation of PRKCQ, a protein kinase C theta isoform involved in various signaling pathways. This antibody, produced in rabbits, exhibits high reactivity towards human samples and has been extensively validated for use in Western blot applications. By targeting the phosphorylated form of PRKCQ at serine 676, this antibody allows for the detection and quantification of PRKCQ activation in diverse cell types, making it an invaluable tool for studies in immunology and cancer research.
PRKCQ is a crucial player in immune cell activation and regulation, being implicated in T cell receptor signaling and cytokine production. Dysregulation of PRKCQ activity has been linked to various autoimmune diseases, inflammatory disorders, and cancer progression, highlighting its potential as a therapeutic target. By understanding the phosphorylation status of PRKCQ at S676, researchers can gain insights into the activation state of this kinase and its impact on immune responses, paving the way for the development of targeted therapies for immune-related conditions.
Product Name:
Phospho-PRKCQ-S676 Polyclonal Antibody
SKU:
CABP0260
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A phospho specific peptide corresponding to residues surrounding S676 of human PRKCQ
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors.
Purification Method:
Affinity purification
Gene ID:
5588
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of Jurkat, using Phospho-PRKCQ-S676 antibody (CABP0260) at 1:1000 dilution.Jurkat cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
