The Phospho-PRKCA (S657/Y658) Rabbit Polyclonal Antibody (CABP0970) is a specialized tool for researchers studying protein kinase C alpha (PRKCA) signaling pathways. This antibody is raised in rabbits and specifically recognizes the phosphorylated form of PRKCA at serine 657 and tyrosine 658 residues. PRKCA is a key enzyme involved in various cellular processes, including cell growth, differentiation, and apoptosis. Phosphorylation of PRKCA at S657/Y658 has been linked to its activation and modulation of downstream signaling cascades. This antibody enables the detection and analysis of phosphorylated PRKCA in different cell types, providing valuable insights into its role in cellular signaling events.
Validated for use in Western blot applications, the Phospho-PRKCA (S657/Y658) Rabbit Polyclonal Antibody is a valuable tool for researchers in the fields of cell biology, cancer research, and signal transduction. By studying the phosphorylation status of PRKCA, researchers can gain a deeper understanding of its function and potential implications in disease processes.
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
Purification Method:
Affinity purification
Gene ID:
5578
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-PKC alpha-S657/Y658 Rabbit pAb (CABP0970) at 1:1000 dilution.293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.293T cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
