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Phospho-PKC alpha-S657/Y658 Rabbit Polyclonal Antibody (CABP0970)

SKU:
CABP0970
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cardiovascular
€419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-PKC alpha-S657/Y658 Rabbit Polyclonal Antibody
SKU:CABP0970
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around S657 & Y658 of human PKC alpha (NP_002728.2).
Sequence:GFSY VN
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:AAG6; PKCA; PRKACA; PKCI+/-; PKCalpha; PKC-alpha; Phospho-PKC alpha-S657/Y658
Positive Sample:293T+CIP,293T,293T+PMA
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm, Mitochondrion membrane, Nucleus, Peripheral membrane protein.
Calculated MW:77kDa
Observed MW:82kDa

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

Purification Method:Affinity purification
Gene ID:5578
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Phospho-PKC alpha-S657/Y658 Rabbit pAb (CABP0970) at 1:1000 dilution.293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.293T cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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