The Phospho-PRKACA-T197 Polyclonal Antibody (CABP0557) is specifically designed for research involving the phosphorylation of PRKACA at threonine 197, a key regulatory site in the protein kinase A signaling pathway. This antibody, raised in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications.PRKACA, or protein kinase A catalytic subunit alpha, is a crucial enzyme involved in various cellular processes, including cell proliferation, differentiation, and metabolism. Phosphorylation at threonine 197 is known to modulate the activity of PRKACA, thereby influencing downstream signaling pathways associated with cell growth and survival.
The Phospho-PRKACA-T197 Polyclonal Antibody enables specific detection and analysis of the phosphorylated form of PRKACA in different cell types, making it an essential tool for studies in molecular biology, cell signaling, and cancer research. Understanding the regulation of PRKACA phosphorylation at threonine 197 is key to unraveling its role in normal cellular functions and disease states, paving the way for the development of targeted therapies for various human disorders.
This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms.
Purification Method:
Affinity purification
Gene ID:
5566
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of 293T cells, using Phospho-PKA C-alpha (PRKACA)-T197 antibody (CABP0557) at 1:1000 dilution. 293T cells were treated by Insulin (100nM) for 10 minutes or treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
