The Phospho-PRKACA (S339) Polyclonal Antibody (CABP0558) is a crucial tool for researchers studying PRKACA phosphorylation at serine 339. This antibody, generated in rabbits, exhibits strong reactivity with human samples and is validated for use in Western blotting applications. By specifically binding to the phosphorylated form of PRKACA at serine 339, this antibody enables accurate detection and analysis of PRKACA activity in a variety of cell types.PRKACA, also known as protein kinase A catalytic subunit alpha, plays a key role in numerous cellular processes, including cell growth, differentiation, and metabolism. Phosphorylation of PRKACA at serine 339 is known to regulate its activity and downstream signaling pathways, making this antibody an invaluable tool for research in signal transduction, cancer biology, and metabolic disorders.
Understanding the role of PRKACA phosphorylation at serine 339 is essential for unraveling its complex functions in cellular physiology and disease pathology. By using this antibody, researchers can delve deeper into the mechanisms underlying PRKACA-mediated signaling and develop targeted therapies for conditions linked to dysregulated PRKACA activity, such as cancer, diabetes, and cardiovascular diseases.
This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms.
Purification Method:
Affinity purification
Gene ID:
5566
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa and 293 cells, using Phospho-PKA C-alpha (PRKACA)-S339 Rabbit pAb (CABP0558) at 1:1000 dilution. HeLa cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight.293T cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
