The PRKACA Polyclonal Antibody (CAB0798) is a valuable tool for researchers studying the PRKACA protein, which is a key component of the PKA signaling pathway. This antibody, produced in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blot applications. By targeting the PRKACA protein, this antibody enables precise detection and analysis in a variety of cell types, making it well-suited for investigations in cell signaling, metabolism, and cancer biology.PRKACA plays a crucial role in regulating various cellular processes, including gene transcription, metabolism, and cell proliferation.
Dysregulation of PRKACA activity has been implicated in numerous diseases, such as cancer, diabetes, and cardiovascular disorders. Therefore, elucidating the function of PRKACA is essential for uncovering potential therapeutic targets and developing novel treatment strategies for these conditions.In conclusion, the PRKACA Polyclonal Antibody is a reliable tool for researchers seeking to explore the function and mechanisms of PRKACA in health and disease. Its specificity and sensitivity make it an invaluable asset for studies in molecular biology, pharmacology, and drug discovery.
Product Name:
PKA C-alpha (PRKACA) Rabbit Polyclonal Antibody
SKU:
CAB0798
Size:
20uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-351 of human PKA C-alpha (PRKACA) (NP_002721.1).
This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms.
Purification Method:
Affinity purification
Gene ID:
5566
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using PKA C-alpha (PRKACA) Rabbit pAb (CAB0798) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
