The Phospho-PRKAA1 (T183) / PRKAA2 (T172) Polyclonal Antibody (CABP0432) is a valuable tool for researchers studying the PRKAA1 and PRKAA2 proteins, which play essential roles in cellular energy regulation and metabolism. This antibody, produced in rabbits, exhibits high specificity for human samples and has been validated for use in Western blot applications. By binding specifically to the phosphorylated forms of PRKAA1 and PRKAA2, this antibody enables precise detection and analysis in a variety of cell types.PRKAA1 and PRKAA2, also known as AMP-activated protein kinases, serve as key sensors of cellular energy status and play crucial roles in regulating processes such as glucose uptake, lipid metabolism, and protein synthesis.
Dysregulation of PRKAA1 and PRKAA2 activity has been linked to various diseases, including metabolic disorders, cancer, and neurodegenerative diseases. Studying the phosphorylation status of PRKAA1 and PRKAA2 is essential for understanding their functions and developing potential therapeutic interventions targeting these pathways.By utilizing the Phospho-PRKAA1 (T183) / PRKAA2 (T172) Polyclonal Antibody (CABP0432) in your research, you can gain valuable insights into the complex mechanisms underlying cellular energy regulation and metabolism, paving the way for advancements in fields such as metabolism,
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Purification Method:
Affinity purification
Gene ID:
5562/5563
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells, using Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (CABP0432) at 1:1000 dilution.C6 cells were treated by AICAR (0.5 mM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
