null

Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit Polyclonal Antibody (CABP0432)

SKU:
CABP0432
Product Type:
Antibody
Applications:
WB
IHC
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
€419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit Polyclonal Antibody
SKU:CABP0432
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around T183 of human PRKAA1 (NP_006242.5).
Sequence:LRTS C
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:AMPKa1/AMPKa2; Phospho-AMPKa1-T183/AMPKa2-T172
Positive Sample:C6
Conjugate:Unconjugated
Cellular Localization:apical plasma membrane, cytoplasm, cytosol, nuclear speck, nucleoplasm, nucleus
Calculated MW:64kDa/65kDa/62kDa
Observed MW:64kDa

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Purification Method:Affinity purification
Gene ID:5562/5563
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from C6 cells, using Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (CABP0432) at 1:1000 dilution.C6 cells were treated by AICAR (0.5 mM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Related Products