The Phospho-PPP2CA/PPP2CB (Y307) Rabbit Polyclonal Antibody (CABP0927) is an essential tool for researchers studying the phosphorylation of PPP2CA and PPP2CB proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity in detecting phosphorylated Y307 residues of PPP2CA and PPP2CB in human samples.Validation studies have shown that this antibody is suitable for Western blot applications, enabling precise analysis of the phosphorylation status of PPP2CA and PPP2CB in various cell types. The ability to target specific phosphorylation sites on these proteins makes this antibody particularly useful for investigations in signal transduction pathways, protein regulation, and cell signaling.
PPP2CA and PPP2CB are key proteins involved in cellular processes such as cell cycle regulation, DNA repair, and apoptosis. Phosphorylation of these proteins at the Y307 site is known to play a crucial role in modulating their activity and function. By using this antibody, researchers can gain valuable insights into the regulatory mechanisms of PPP2CA and PPP2CB, which may have implications in cancer biology, neurodegenerative diseases, and other pathological conditions.Overall, the Phospho-PPP2CA/PPP2CB (Y307) Rabbit Polyclonal Antibody (CABP0927) serves as an indispensable tool for studying the phosphorylation events of PPP2CA and PPP2CB proteins, offering researchers a deeper understanding of their
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]
Purification Method:
Affinity purification
Gene ID:
5515/5516
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-PPP2CA/PPP2CB-Y307 Rabbit pAb (CABP0927) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
