The Phospho-PPP1R12A (T853) Rabbit Polyclonal Antibody (CABP0916) is a highly specific tool for studying the phosphorylation of the PPP1R12A protein at threonine 853. This antibody, raised in rabbits, has been validated for use in Western blot applications and is highly reactive with human samples.PPP1R12A is a regulatory subunit of protein phosphatase 1 (PP1) involved in the regulation of smooth muscle contraction and cell motility. Phosphorylation at threonine 853 plays a crucial role in modulating the activity of PPP1R12A and affecting its functions in cellular processes.
By using the Phospho-PPP1R12A (T853) Rabbit Polyclonal Antibody, researchers can investigate the phosphorylation status of PPP1R12A and its impact on smooth muscle physiology, cell motility, and other cellular functions. This antibody provides a valuable tool for studying signaling pathways involving PPP1R12A and exploring its role in diseases such as cancer, cardiovascular disorders, and smooth muscle-related conditions.
Product Name:
Phospho-PPP1R12A-T853 Rabbit Polyclonal Antibody
SKU:
CABP0916
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T853 of human PPP1R12A (NP_002471.1).
Sequence:
RSTG V
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
MBS; GUBS; M130; MYPT1; Phospho-PPP1R12A-T853
Positive Sample:
HeLa,NIH/3T3
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm.
Calculated MW:
115kDa
Observed MW:
125kDa
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
4659
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-PPP1R12A-T853 Rabbit pAb ( AP0916) at 1:1000 dilution.Hela cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
