The Phospho-PPP1R12A (S668) Polyclonal Antibody (CABP0835) is a valuable tool for researchers studying the phosphorylation of PPP1R12A at serine 668. This antibody, raised in rabbits, is highly specific for detecting the phosphorylated form of PPP1R12A in human samples and is validated for use in Western blot applications.PPP1R12A is a key regulatory subunit of protein phosphatase 1 (PP1) that plays a crucial role in the regulation of actomyosin contractility. Phosphorylation of PPP1R12A at serine 668 is known to modulate its activity, impacting processes such as smooth muscle contraction and cell migration. The Phospho-PPP1R12A (S668) Polyclonal Antibody allows for the detection and quantification of this phosphorylation event, facilitating research into the signaling pathways and biological functions regulated by PPP1R12A.
This antibody is an essential tool for investigating the role of PPP1R12A phosphorylation in various cellular processes, making it ideal for studies in cell biology, signaling pathways, and diseases related to dysregulated actomyosin contractility. Researchers interested in understanding the molecular mechanisms underlying these physiological and pathological processes will find the Phospho-PPP1R12A (S668) Polyclonal Antibody to be a valuable asset in their research endeavors.
Product Name:
Phospho-PPP1R12A-S668 Rabbit Polyclonal Antibody
SKU:
CABP0835
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S668 of human PPP1R12A (NP_001137357.1).
Sequence:
RRSY L
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
MBS; GUBS; M130; MYPT1; Phospho-PPP1R12A-S668
Positive Sample:
HeLa,C6
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm.
Calculated MW:
115kDa
Observed MW:
160kDa
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
4659
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-PPP1R12A-S668 Rabbit pAb (CABP0835) at 1:2000 dilution. Both HeLa cells and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
