The Phospho-PPP1R12A (S507) Polyclonal Antibody (CABP0769) is specifically designed for research involving PPP1R12A, a protein involved in the regulation of smooth muscle contraction and cell movement. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. By targeting the phosphorylated form of PPP1R12A at serine 507, researchers can investigate the phosphorylation status of this critical protein and its role in cellular processes.PPP1R12A, also known as MYPT1, is a key regulator of myosin phosphatase activity and smooth muscle contraction. Phosphorylation of PPP1R12A at serine 507 is known to modulate its function, affecting cytoskeletal dynamics and cell migration.
Understanding the phosphorylation status of PPP1R12A is essential for deciphering its role in cell movement, tissue remodeling, and disease progression.This antibody provides a valuable tool for studying the phosphorylation of PPP1R12A and its impact on cellular functions. Its application in Western blot analysis allows for the detection and quantification of phosphorylated PPP1R12A in various cell types and tissues, making it an ideal choice for research in cell biology, neuroscience, and cardiovascular disease. By investigating the phosphorylation of PPP1R12A, researchers can gain insights into the mechanisms underlying smooth muscle regulation and potentially identify new therapeutic
Product Name:
Phospho-PPP1R12A-S507 Rabbit Polyclonal Antibody
SKU:
CABP0769
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S507 of human PPP1R12A (NP_001137357.1).
Sequence:
LAST S
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
MBS; GUBS; M130; MYPT1; Phospho-PPP1R12A-S507
Positive Sample:
HeLa,Jurkat
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm.
Calculated MW:
115kDa
Observed MW:
135kDa
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
4659
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-PPP1R12A-S507 Rabbit pAb (CABP0769) at 1:2000 dilution or PPP1R12A antibody (CAB0587). HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
