The Phospho-PPP1R12A/PPP1R12B/PPP1R12C (T696) Rabbit Polyclonal Antibody (CABP1164) is a valuable tool for researchers studying the phosphorylation of PPP1R12A, PPP1R12B, and PPP1R12C proteins. These proteins play crucial roles in cellular processes, including cell proliferation, cytoskeletal organization, and smooth muscle contraction.This antibody, raised in rabbits, is highly specific and sensitive, making it ideal for use in Western blot and immunohistochemistry applications. It binds specifically to the phosphorylated T696 residue on PPP1R12A, PPP1R12B, and PPP1R12C, allowing for the accurate detection and quantification of these phosphorylated proteins in various experimental settings.
The phosphorylation of PPP1R12A, PPP1R12B, and PPP1R12C at the T696 residue is known to regulate their activity and function, making this antibody an essential tool for researchers investigating the signaling pathways and cellular processes influenced by these proteins. Its high reactivity with human samples further expands its utility in studies related to cell signaling, cancer biology, and smooth muscle function.Overall, the Phospho-PPP1R12A/PPP1R12B/PPP1R12C (T696) Rabbit Polyclonal Antibody (CABP1164) provides researchers with a reliable and specific tool
A synthetic phosphorylated peptide around T696 of human PPP1R12A/PPP1R12B/PPP1R12CPPP1R12A (NP_002471.1).
Sequence:
RSTQ G
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Positive Sample:
Jurkat
Cellular Localization:
A band, actin cytoskeleton, centrosome, contractile fiber, cytoplasm, cytosol, nucleolus, nucleoplasm, plasma membrane, Z disc
Observed MW:
140kDa
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]
Purification Method:
Affinity purification
Gene ID:
4659/4660/54776
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using Phospho-PPP1R12A/PPP1R12B/PPP1R12C-T696 Rabbit pAb (CABP1164) at 1:1000 dilution.Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
