The Phospho-PPP1CA (T320) Polyclonal Antibody (CABP0786) is a valuable tool for researchers studying the phosphorylation of PPP1CA, a key regulatory subunit of protein phosphatase 1 (PP1). This antibody, raised in rabbits, is highly specific for the phosphorylated form of PPP1CA at threonine 320 and is validated for use in Western blot applications. By targeting this specific phosphorylation site, researchers can investigate the signaling pathways and cellular processes regulated by PPP1CA in various biological contexts.PPP1CA is known to be involved in the regulation of a wide range of cellular functions, including cell cycle progression, gene expression, and protein synthesis. Phosphorylation of PPP1CA at threonine 320 has been suggested to play a role in modulating its enzymatic activity and substrate specificity, making it a crucial event in the regulation of PP1-mediated signaling pathways.
By using this antibody to detect and study phospho-PPP1CA, researchers can gain valuable insights into the role of this phosphatase in cell signaling and its potential implications in disease processes.Overall, the Phospho-PPP1CA (T320) Polyclonal Antibody (CABP0786) is a powerful tool for investigating the phosphorylation status and functional dynamics of PPP1CA in various research settings, making it an essential reagent for studies in cell biology, signal transduction, and disease
Product Name:
Phospho-PPP1CA-T320 Rabbit Polyclonal Antibody
SKU:
CABP0786
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T320 of human PPP1CA (NP_002699.1).
Sequence:
PITP P
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB,1:500 - 1:2000 IHC-P,1:50 - 1:100
Synonyms:
PP1A; PP-1A; PPP1A; PP1alpha; Phospho-PPP1CA-T320
Positive Sample:
C6,NIH/3T3,HeLa
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus, nucleolus, nucleoplasm.
Calculated MW:
38kDa
Observed MW:
38kDa
The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). This broadly expressed gene encodes the alpha subunit of the PP1 complex that associates with over 200 regulatory proteins to form holoenzymes which dephosphorylate their biological targets with high specificity. PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies suggest that PP1 is an important regulator of cardiac function and that PP1 deregulation is implicated in diabetes and multiple types of cancer. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5499
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells using Phospho-PPP1CA-T320 Rabbit pAb (CABP0786) at 1:500 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
