The Phospho-PPIG (S376) Polyclonal Antibody (CABP0842) is a crucial tool for researchers studying the phosphorylation of PPIG, a protein involved in various cellular processes such as cell proliferation and survival. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the phosphorylated form of PPIG at Serine 376, this antibody allows for the detection and analysis of PPIG phosphorylation levels in different cell types.PPIG is known to play a key role in signaling pathways that regulate cell growth and survival, making it a potential target for cancer research.
The phosphorylation of PPIG at Serine 376 has been implicated in modulating its activity and function, suggesting its importance in various cellular functions. Understanding the phosphorylation status of PPIG can provide valuable insights into the mechanisms underlying cancer development and progression, as well as other diseases where PPIG is involved.Overall, the Phospho-PPIG (S376) Polyclonal Antibody (CABP0842) is a reliable tool for researchers looking to study the role of PPIG phosphorylation in cellular processes and diseases, providing essential information for the development of novel therapeutic strategies targeting PPIG and its signaling pathways.
Product Name:
Phospho-PPIG-S376 Rabbit Polyclonal Antibody
SKU:
CABP0842
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S376 of human PPIG (NP_004783.2).
Sequence:
VSSG E
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
CYP; SRCyp; SCAF10; CARS-Cyp; Phospho-PPIG-S376
Positive Sample:
293T,C2C12,PC-12
Conjugate:
Unconjugated
Cellular Localization:
Nucleus matrix, Nucleus speckle.
Calculated MW:
89kDa
Observed MW:
110kDa
Enables cyclosporin A binding activity and peptidyl-prolyl cis-trans isomerase activity. Involved in protein peptidyl-prolyl isomerization. Located in cytosol and nuclear speck.
Purification Method:
Affinity purification
Gene ID:
9360
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-PPIG-S376 Rabbit pAb (CABP0842) at 1:1000 dilution. 293T cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour. C2C12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
