The Phospho-PP2A Alpha (Y307) Rabbit Monoclonal Antibody (CABP1043) is a valuable tool for researchers studying the phosphorylation of PP2A alpha, a key regulator of cell growth and division. This antibody, produced in rabbits, is highly specific to the phosphorylated form of PP2A alpha at tyrosine 307 and is suitable for use in Western blot applications.PP2A alpha is a well-known serine/threonine phosphatase that plays a critical role in cell signaling pathways by dephosphorylating key protein substrates. Phosphorylation of PP2A alpha at tyrosine 307 has been linked to its activation and regulation of downstream signaling cascades.
This antibody enables the detection and analysis of phosphorylated PP2A alpha in various cell types, making it an essential tool for studies in cancer research, cell biology, and signal transduction pathways.Understanding the regulation of PP2A alpha phosphorylation at tyrosine 307 is crucial for unraveling its role in cell growth, survival, and proliferation. This antibody provides researchers with a valuable tool to explore the intricate mechanisms underlying PP2A alpha signaling and its implications in various cellular processes.
Chromosome, Cytoplasm, Nucleus, centromere, cytoskeleton, spindle pole.
Calculated MW:
36kDa
Observed MW:
35kDa
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.
Purification Method:
Affinity purification
Gene ID:
5515
Clone Number:
ARC1589
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PP2A Catalytic α-Y307 Rabbit mAb (CABP1043) at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
