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Phospho-PP2A Catalytic α-Y307 Rabbit Monoclonal Antibody (CABP1043)

SKU:
CABP1043
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cell Biology
€419
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Description

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Product Name:Phospho-PP2A Catalytic α-Y307 Rabbit Monoclonal Antibody
SKU:CABP1043
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:A synthetic phosphorylated peptide around Y307 of human PP2A Catalytic α (P67775).
Sequence:PDYF L
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:RP-C; PP2Ac; PP2CA; NEDLBA; PP2Calpha; Phospho-PP2A Catalytic α-Y307
Positive Sample:NIH/3T3（Forskolin ，30 uM ）
Conjugate:Unconjugated
Cellular Localization:Chromosome, Cytoplasm, Nucleus, centromere, cytoskeleton, spindle pole.
Calculated MW:36kDa
Observed MW:35kDa

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.

Purification Method:Affinity purification
Gene ID:5515
Clone Number:ARC1589
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of NIH/3T3 cells, using Phospho-PP2A Catalytic α-Y307 Rabbit mAb (CABP1043) at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.

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