The Phospho-POU2F1/POU2F2 (S385) Antibody (PACO04644) is a valuable tool for researchers studying the phosphorylation of POU2F1/POU2F2 proteins. These transcription factors play a vital role in gene regulation and are involved in various cellular processes, including development, differentiation, and proliferation. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it suitable for Western blot applications. It specifically recognizes the phosphorylated form of POU2F1/POU2F2 at the serine 385 residue, allowing for precise detection and quantification in different cell types.The phosphorylation of POU2F1/POU2F2 at S385 is known to influence their transcriptional activity and can impact gene expression patterns.
Therefore, this antibody is essential for investigating the functional consequences of this post-translational modification in cellular signaling pathways and disease processes.Researchers studying signal transduction, cell signaling, and transcriptional regulation will find this antibody particularly useful in dissecting the molecular mechanisms underlying various physiological and pathological conditions. Its versatility and reliability make it a valuable asset in advancing our understanding of POU2F1/POU2F2-dependent processes and potential therapeutic targets.
Antibody Name:
Phospho-POU2F1/POU2F2 (S385) Antibody
Antibody SKU:
PACO04644
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
IHC:1:100-1:300
Species Reactivity:
Human, Mouse, Rat
Immunogen:
synthesized peptide derived from human 41183 around the phosphorylation site of S385.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Synonyms:
POU2F1; OCT1; OTF1; POU domain; class 2, transcription factor 1; NF-A1; Octamer-binding protein 1; Oct-1; Octamer-binding transcription factor 1; OTF-1; POU2F2; OCT2; OTF2; POU domain, class 2, transcription factor 2; Lymphoid-restricted im
UniProt Protein Function:
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR. In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.
NCBI Summary:
The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM, Jul 2010]
