The Phospho-POLR2A (S5) Rabbit Monoclonal Antibody (CABP0997) is a powerful tool for studying the phosphorylation of POLR2A at serine 5, a key event in transcription regulation. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting phosphorylated POLR2A in human samples, making it an excellent choice for Western blot applications.Phosphorylation of POLR2A at serine 5 is a critical step in the transcription process, influencing RNA polymerase II activity and gene expression. By targeting this specific phosphorylation site, researchers can gain valuable insights into the molecular mechanisms underlying gene regulation and transcriptional control.
The Phospho-POLR2A (S5) Rabbit Monoclonal Antibody is an essential tool for researchers in the fields of molecular biology, biochemistry, and genetics. Its ability to selectively recognize phosphorylated POLR2A at serine 5 makes it an invaluable asset for studying transcriptional dynamics and deciphering the complexities of gene expression regulation.
Product Name:
Phospho-POLR2A-S5 Rabbit Monoclonal Antibody
SKU:
CABP0997
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A phospho specific peptide corresponding to residues surrounding S5 of human POLR2A CTD (P24928).
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
Purification Method:
Affinity purification
Gene ID:
5430
Clone Number:
ARC1541
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-POLR2A CTD-S5 Rabbit mAb (CABP0997) at 1:1000 dilution.MCF7 cells were treated by Doxorubicin (0.5 uM) at 37℃ for 24 hours or treated by nocodazole (50 ng/mL) at 37℃ for 20 hours or treated by CIP(20uL/400ul) at 37℃ for 1 hour.C2C12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
