The Phospho-PolR2A (S5) Polyclonal Antibody (CABP0828) is designed for research involving the phosphorylated form of PolR2A, a subunit of RNA polymerase II that plays a crucial role in transcription regulation. This antibody, raised in rabbits, is highly specific for the phosphorylated form of PolR2A at Serine 5 and is validated for use in Western blot applications. It binds specifically to the phosphorylated PolR2A protein, allowing for accurate detection and analysis in a variety of cell types, making it an ideal tool for studies in molecular biology and gene regulation.Phosphorylation of PolR2A at Serine 5 is known to be involved in the regulation of gene expression, particularly during the early stages of transcription.
Understanding the role of this phosphorylation event is essential for researchers studying mechanisms of transcriptional control, gene expression, and RNA synthesis. The Phospho-PolR2A (S5) Polyclonal Antibody provides a valuable tool for investigating the function and regulation of PolR2A phosphorylation in various biological processes, making it a key component in studies related to transcriptional dynamics and gene regulation.
Product Name:
Phospho-POLR2A-S5 Rabbit Polyclonal Antibody
SKU:
CABP0828
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A phospho specific peptide corresponding to residues surrounding S5 of human POLR2A CTD repeat YSPTSPS.
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
Purification Method:
Affinity purification
Gene ID:
5430
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-POLR2A CTD-S5 Rabbit pAb (CABP0828) at 1:1000 dilution.MCF7 and C2C12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
