The Phospho-Polr2a (S2) Polyclonal Antibody (CABP0749) is a valuable tool for researchers studying the phosphorylation of Polr2a, a key component of the RNA polymerase II complex. This antibody is generated in rabbits and is highly reactive with human, mouse, and rat samples, making it versatile for use in various experimental settings.Polr2a is known to be phosphorylated at the S2 site, which is important for the transcriptional activity of RNA polymerase II. By targeting this specific phosphorylation site, researchers can investigate the regulation of gene expression and the impact of Polr2a phosphorylation on cellular processes.
The Phospho-Polr2a (S2) Polyclonal Antibody is validated for use in Western blot and immunohistochemistry applications, allowing for precise detection and visualization of the phosphorylated form of Polr2a in different biological samples. Its high specificity and sensitivity make it an essential tool for studies in transcriptional regulation, gene expression, and various disease states where Polr2a phosphorylation may be dysregulated.
Product Name:
Phospho-POLR2A-S2 Rabbit Polyclonal Antibody
SKU:
CABP0749
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A phospho specific peptide corresponding to residues surrounding S2 of human POLR2A CTD repeat YSPTSPS.
Sequence:
SYSP T
Tested Applications:
WBIPELISA
Recommended Dilution:
WB,1:1000 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
Purification Method:
Affinity purification
Gene ID:
5430
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using Phospho-POLR2A CTD-S2 Rabbit pAb (CABP0749) at 1:1000 dilution. 293T cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour or treated by Doxorubicin (0.5uM) for 24 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
