Phospho-POLR2A CTD-T4 Polyclonal Antibody (CAB21190)
- SKU:
- CAB21190
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
- IP
Frequently bought together:
Description
Product Name: | Phospho-POLR2A CTD-T4 Polyclonal Antibody |
SKU: | CAB21190 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | A phospho specific peptide corresponding to residues surrounding T4 of human POLR2A CTD repeat YSPTSPS. |
Tested Applications: | WB IP ChIP ELISA |
Recommended Dilution: | WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsChIP,5μg antibody for 10μg-15μg of Chromatin |
Synonyms: | RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; NEDHIB; RpIILS; hsRPB1; hRPB220; Phospho-POLR2A CTD-T4 |
Positive Sample: | MCF7,NIH/3T3,C2C12,C6,PC-12 |
Conjugate: | Unconjugated |
Cellular Localization: | Nucleus. |
Calculated MW: | 217kDa |
Observed MW: | 250kDa |
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
Purification Method: | Affinity purification |
Gene ID: | 5430 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |