The Phospho-POLR2A CTD T4 Polyclonal Antibody is a valuable tool for researchers studying transcriptional regulation and RNA polymerase activity. This antibody, produced in rabbits, specifically targets the phosphorylated form of POLR2A C-terminal domain (CTD), a key player in RNA synthesis and processing. Validated for use in Western blot and immunohistochemistry applications, this antibody is highly reactive with human samples, allowing for precise detection and analysis of phospho-POLR2A in various cell types. Its specificity for the phosphorylated form of POLR2A makes it an ideal choice for studies in gene expression regulation, RNA splicing, and transcriptional control mechanisms.
The phosphorylation of POLR2A CTD is crucial for the regulation of transcription initiation, elongation, and termination, making this antibody an essential tool for researchers investigating gene expression dynamics and transcriptional signaling pathways. By targeting the phosphorylated form of POLR2A, this antibody enables detailed insights into the molecular mechanisms driving RNA polymerase activity and gene transcription processes.
Product Name:
Phospho-POLR2A CTD-T4 Polyclonal Antibody
SKU:
CAB21190
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A phospho specific peptide corresponding to residues surrounding T4 of human POLR2A CTD repeat YSPTSPS.
Tested Applications:
WBIPChIPELISA
Recommended Dilution:
WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsChIP,5μg antibody for 10μg-15μg of Chromatin
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
Purification Method:
Affinity purification
Gene ID:
5430
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-POLR2A CTD-T4 Rabbit pAb (CAB21190) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
