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Phospho-POLR2A CTD-T4 Polyclonal Antibody (CAB21190)

SKU:
CAB21190
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
IP
€139 - €489
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-POLR2A CTD-T4 Polyclonal Antibody
SKU:CAB21190
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A phospho specific peptide corresponding to residues surrounding T4 of human POLR2A CTD repeat YSPTSPS.
Tested Applications:WB IP ChIP ELISA
Recommended Dilution:WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsChIP,5μg antibody for 10μg-15μg of Chromatin
Synonyms:RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; NEDHIB; RpIILS; hsRPB1; hRPB220; Phospho-POLR2A CTD-T4
Positive Sample:MCF7,NIH/3T3,C2C12,C6,PC-12
Conjugate:Unconjugated
Cellular Localization:Nucleus.
Calculated MW:217kDa
Observed MW:250kDa

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Purification Method:Affinity purification
Gene ID:5430
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Phospho-POLR2A CTD-T4 Rabbit pAb (CAB21190) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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