The Phospho-PLN (T17) Rabbit Polyclonal Antibody (CABP0910) is a valuable tool for researchers studying the phosphorylation of Phospholamban (PLN) at threonine 17. PLN is a key regulator of cardiac muscle contraction and relaxation, making it a crucial target for understanding heart function and disease. This antibody, raised in rabbits, is highly specific for detecting phosphorylated PLN at threonine 17 in human samples, making it ideal for Western blot applications.Phosphorylation of PLN at threonine 17 is known to regulate the activity of the sarcoplasmic reticulum calcium ATPase (SERCA) pump, which plays a vital role in calcium homeostasis in cardiac muscle cells. Dysregulation of PLN phosphorylation has been implicated in various cardiac diseases, including heart failure and arrhythmias.
By using this antibody to study the phosphorylation status of PLN, researchers can gain insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets for treating heart disease.Overall, the Phospho-PLN (T17) Rabbit Polyclonal Antibody (CABP0910) is a valuable tool for investigating the role of PLN phosphorylation in cardiac physiology and pathology. Its high specificity and sensitivity make it a reliable choice for researchers interested in studying heart function and developing novel treatments for cardiovascular diseases.
Product Name:
Phospho-PLN-T17 Rabbit Polyclonal Antibody
SKU:
CABP0910
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T17 of human PLN (NP_002658.1).
Sequence:
ASTI E
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB,1:100 - 1:500 IHC-P,1:50 - 1:200
Synonyms:
PLB; CMD1P; CMH18; Phospho-PLN-T17
Positive Sample:
HeLa
Cellular Localization:
endoplasmic reticulum, mitochondrial membrane, mitochondrion, perinuclear region of cytoplasm
Calculated MW:
6kDa
Observed MW:
24kDa
The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure, and also familial hypertrophic cardiomyopathy.
Purification Method:
Affinity purification
Gene ID:
5350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-PLN-T17 Rabbit pAb (CABP0910) at 1:400 dilution.HeLa cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
