null

Phospho-PLN-T17 Rabbit Polyclonal Antibody (CABP0910)

SKU:
CABP0910
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
€419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-PLN-T17 Rabbit Polyclonal Antibody
SKU:CABP0910
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around T17 of human PLN (NP_002658.1).
Sequence:ASTI E
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:100 - 1:500 IHC-P,1:50 - 1:200
Synonyms:PLB; CMD1P; CMH18; Phospho-PLN-T17
Positive Sample:HeLa
Cellular Localization:endoplasmic reticulum, mitochondrial membrane, mitochondrion, perinuclear region of cytoplasm
Calculated MW:6kDa
Observed MW:24kDa

The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure, and also familial hypertrophic cardiomyopathy.

Purification Method:Affinity purification
Gene ID:5350
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from HeLa cells, using Phospho-PLN-T17 Rabbit pAb (CABP0910) at 1:400 dilution.HeLa cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.

Related Products