The Phospho-PLK1 (T210) Rabbit Monoclonal Antibody (CABP1025) is a valuable tool for researchers studying the Polo-like kinase 1 (PLK1) protein and its phosphorylation at threonine 210. PLK1 is a key regulator of cell cycle progression and has been implicated in various cancer types. This antibody, raised in rabbits, is highly specific for detecting phosphorylated PLK1 at T210 in human samples and is validated for use in Western blot applications.Phosphorylation of PLK1 at T210 is known to be a critical regulatory event in its activation and function, particularly in promoting cell division and progression through mitosis. Understanding the phosphorylation status of PLK1 can provide important insights into its role in cancer development and progression.
This antibody enables researchers to detect and analyze the phosphorylated form of PLK1 in various cell types, making it an essential tool for studies in cancer biology and cell cycle regulation.The Phospho-PLK1 (T210) Rabbit Monoclonal Antibody (CABP1025) is a valuable asset for researchers investigating the role of PLK1 and its phosphorylation at T210 in cancer, cell cycle regulation, and other cellular processes. Its high specificity and sensitivity make it a reliable choice for studying the functional implications of PLK1 phosphorylation and its potential as a therapeutic target in cancer treatment.
Product Name:
Phospho-PLK1-T210 Rabbit Monoclonal Antibody
SKU:
CABP1025
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around T210 of human PLK1 (P53350).
The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy.
Purification Method:
Affinity purification
Gene ID:
5347
Clone Number:
ARC1570
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of HT-29 cells, using Phospho-PLK1-T210 (CABP1025) at 1:1000 dilution.HT-29 cells were treated by nocodazole (100 ng/mL) at 37℃ for 16 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.
