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Phospho-PLK1-T210 Rabbit Monoclonal Antibody (CABP1025)

SKU:
CABP1025
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cell Cycle
€419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-PLK1-T210 Rabbit Monoclonal Antibody
SKU:CABP1025
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around T210 of human PLK1 (P53350).
Sequence:KKTL C
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:PLK; STPK13; Phospho-PLK1-T210
Positive Sample:HT-29 treated by nocodazole
Conjugate:Unconjugated
Cellular Localization:Chromosome, Cytoplasm, Midbody, Nucleus, Centromere, Centrosome, Cytoskeleton, Kinetochore, Microtubule organizing center, Spindle.
Calculated MW:68kDa
Observed MW:68kDa

The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy.

Purification Method:Affinity purification
Gene ID:5347
Clone Number:ARC1570
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of HT-29 cells, using Phospho-PLK1-T210 (CABP1025) at 1:1000 dilution.HT-29 cells were treated by nocodazole (100 ng/mL) at 37℃ for 16 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.

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