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PLK1 Monoclonal Antibody (CAB21082)

SKU:
CAB21082
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
  • Immunohistochemistry of paraffin-embedded rat spleen using PLK1 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse spleen using PLK1 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
  • Immunohistochemistry of paraffin-embedded rat ovary using PLK1 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
  • Immunohistochemistry of paraffin-embedded human tonsil using PLK1 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
  • Immunohistochemistry of paraffin-embedded human esophageal cancer using PLK1 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using PLK1 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
  • Western blot analysis of extracts of various cell lines, using PLK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of extracts of various cell lines, using PLK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
€179 - €469
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Description

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PLK1 Monoclonal Antibody (CAB21082)

The PLK1 Polyclonal Antibody (CAB21082) is a powerful tool for researchers studying the PLK1 protein, a key regulator of cell division and a potential target for cancer therapy. This antibody, developed through rabbit immunization, offers high specificity and sensitivity for detecting PLK1 in human samples, making it ideal for use in Western blot applications.PLK1, or Polo-like kinase 1, plays a crucial role in cell cycle progression and is often overexpressed in cancer cells, promoting uncontrolled cell growth. By targeting PLK1, researchers hope to develop more effective cancer treatments that inhibit tumor cell proliferation.

The PLK1 Polyclonal Antibody enables precise detection and analysis of PLK1 expression levels in various cell types, providing valuable insights for cancer research and drug development efforts.Understanding the function and regulation of PLK1 is essential for identifying novel therapeutic strategies for treating cancer and other diseases characterized by abnormal cell division. The PLK1 Polyclonal Antibody serves as a valuable tool for investigating the role of PLK1 in cell biology and disease progression, paving the way for potential breakthroughs in cancer treatment.

Product Name:PLK1 Monoclonal Antibody
SKU:CAB21082
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat,Monkey
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 272-495 of human PLK1 (NP_005021.2).
Sequence:KHIN PVAA SLIQ KMLQ TDPT ARPT INEL LNDE FFTS GYIP ARLP ITCL TIPP RFSI APSS LDPS NRKP LTVL NKGL ENPL PERP REKE EPVV RETG EVVD CHLS DMLQ QLHS VNAS KPSE RGLV RQEE AEDP ACIP IFWV SKWV DYSD KYGL GYQL CDNS VGVL FNDS TRLI LYND GDSL QYIE RDGT ESYL TVSS HPNS LMKK ITLL KYFR NYMS EHLL KAGA
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:PLK; STPK13; PLK1
Positive Sample:HeLa,Jurkat,K-562,COS-7,Mouse testis,Rat testis
Conjugate:Unconjugated
Cellular Localization:Chromosome, Cytoplasm, Midbody, Nucleus, centromere, centrosome, cytoskeleton, kinetochore, microtubule organizing center, spindle.
Calculated MW:68kDa
Observed MW:62kDa

The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy.

Purification Method:Affinity purification
Gene ID:5347
Clone Number:ARC52311
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using PLK1 antibody (CAB21082) at1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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